Table 4.
Unidirectional and net fluxes of Na* and Cl− across distal colon of hyper-aldosteronaemic rats pre-exposed to cholera toxin:effect of butyrate and bumetanide
| G | ISC | JNa,ms | JNa,sm | JNa,net | JCl,ms | JCl,sm | JCl,net | |
|---|---|---|---|---|---|---|---|---|
| Control | ||||||||
| Post-CT | 9.7 ± 0.6 | 3.2 ± 0.2 | 7.4 ± 0.7 | 4.3 ± 0.6 | 3.2 ± 0.4 | 12.2 ± 0.7 | 13.4 ± 0.7 | −1.2 ± 0.5 |
| Bumetanide | 11.5 ± 0.6§ | 1.1 ± 0.1§ | 8.2 ± 0.9n.s. | 5.3 ± 0.8* | 3.0 ± 0.9n.s. | 13.8 ± 0.9* | 10.1 ± 0.8* | 3.7 ± 1.0‡ |
| Butyrate | ||||||||
| Post-CT | 9.0 ± 0.5n.s. | 1.3 ± 0.1§ | 10.2 ± 1.2n.s. | 4.1 ± 0.5n.s. | 6.2 ± 0.9* | 19.3 ± 1.4‡ | 12.7 ± 0.7n.s. | 6.6 ± 1.0‡ |
| Bumetanide | 10.3 ± 0.5† | 0.8 ± 0.1§ | 11.1 ± 0.8n.s. | 5.6 ± 0.8† | 5.6 ± 0.8n.s. | 20.2 ± 1.1n.s. | 12.9 ± 0.8n.s. | 7.3 ± 0.6n.s. |
Values shown are means ±s.e.m. of matched tissue pairs;control,n =8 pairs and butyrate,n =9 pairs. Bumetanide was always added to the serosal reservoir after three flux periods. See Table 1 for further details and definitions.