Figure 2. Effect of NFA on I_Cl(Ca) stimulated by 500 nm[Ca²⁺]_i.

A, whole-cell current recorded from a typical rabbit pulmonary artery myocyte at a holding potential (V_h) of −50 mV. NFA (100 μm) was applied to the cell as indicated by the filled bar, the dotted line represents zero current. B, recording of current from a second pulmonary artery cell where depolarising voltage ramps from −80 to +80 mV were applied at 20 s intervals from V_h of −50 mV. The cell was exposed to NFA (100 μm) as indicated by the filled bar, dotted line represents zero current. C, plot of current recorded during the indicated voltage ramps from the cell shown in B against voltage before (a, Control), during the application of NFA (b, NFA) and on wash out (c, Wash). Note that NFA increased I_Cl(Ca) at negative potentials but decreased the current at positive potentials.