Table 1.
Effect of NFA (100 μm) on the kinetic properties of ICl(Ca)activated by 500μm or 1μm[Ca2+]i in voltage jump experiments in rabbit pulmonary artery smooth muscle cells
| +70mV | −80mV | |||||||
|---|---|---|---|---|---|---|---|---|
| Iinst+70(pA) | (n) | Irelax+70(pA) | (n) | Iinst−80(pA) | (n) | Irelax−80(pA) | (n) | |
| 500nm[Ca2+]i | ||||||||
| Control | 153 ± 21 | (26) | 121 ± 21 | (26) | 470 ± 69 | (26) | 173 ± 25 | (26) |
| +NFA | 159 ± 15 | (26) | 12 ± 2 | (26)* | 259 ± 23 | (26)* | 24 ± 3 | (26)* |
| Wash | 327 ± 49 | (17)* | 55 ± 9 | (17)* | 555 ± 62 | (17) | 80 ± 18 | (17)* |
| 1 μ[Ca2+]i | ||||||||
| Control | 296 ± 24 | (11)† | 76 ± 16 | (11)† | 492 ± 46 | (11) | 92 ± 15 | (11)† |
| +NFA | 158 ± 45 | (11)* | 9 ± 2 | (11)* | 246 ± 56 | (11)* | 18 ± 5 | (11)* |
| Wash | 272 ± 28 | (6) | 33 ± 9 | (6)* | 324 ± 67 | (6) | 36 ± 10 | (6)* |
The definitions of the current parameters are shown in Fig.6A and n values are shown in parentheses.
*
Indicates significant difference (P < 0.05) from control as determined by Student's paired t test
†
indicates significant difference (P < 0.05) from 500 μm[Ca2+]i as determined by Student's unpaired ttest.