Table 2.
Influence of the ion composition on glutamine transport
| NaCl(mm) | Replacement salts(mm) | KCl | Transport activity (pmol glutamine(10 min)−1oocyte−1) |
|---|---|---|---|
| 82.5 | — | 2.5 | 78 ±11a |
| 0 | LiCl (82.5) | 2.5 | 51 ± 9a |
| 0 | Choline choride(82.5) | 2.5 | 4.4 ± 0.6a |
| 0 | Sodium gluconate(82.5) | 0 | 69 ± 12a |
| 82.5 | — | 0 | 99 ± 16b |
| 82.5 | — | 0.3 | 95 ± 18b |
| 82.5 | — | 1 | 100 ± 15b |
| 82.5 | — | 3 | 108 ± 16b |
| 82.5 | — | 10 | 88 ± 12b |
| 82.5 | — | 30 | 80 ± 14b |
Oocytes were injected with 20 ng SN1 cRNA or remained uninjected. After an expression period of 3 days uptake of [14C]glutamine (0.1 mm) was determined over an incubation period of 10 min in oocyte ringer OR2+ of the indicated modified ion composition. The table contains data from independent sets of experiments that are indicated by superscripts a and b. The transport activity of non-injected oocytes is already subtracted.