Figure 1. Refilling of the RRP is faster at the higher temperature.

A, example trace of paired hypertonically mediated responses at 25 and 35 °C. Bars represent 3 s applications of 500 mm sucrose. Interpulse intervals are 1, 4, 7, 10, 13 and 16 s. Additionally, the total time of one episode (paired pulse) was between 30 and 60 s in order to allow adequate time for refilling of the RRP. Since refilling was significantly faster at the higher temperature, additional interpulse interval times (0.5, 1.5, 2, 2.5, 3 and 3.5 s) were also examined at 35 °C in order to obtain better resolution of the fast component of refilling (raw traces not shown). B, the degree to which the charge of the second response (A2) had recovered relative to the charge of the first response (A1) was plotted [1 - (A1 - A2)/A1] as a function of the interpulse interval time for both 35 (○) and 25 °C (•). The data were best fitted by biexponential curves, indicating that refilling of the RRP involves both a fast and a slow component (n = 9–24 for each temperature point).