Figure 6. Representative tracing showing inhibition of ACh-induced contraction by ML-9 (a), Y-27632 (b), dibutyryl cAMP (db-cAMP; c) and 8-bromo-cGMP (8-Br-cGMP; d).

The contraction was induced by adding 100 μm ACh, then 30 μm ML-9, 1 μm Y-27632, 200 μm db-cAMP and 200 μm 8-Br-cGMP was applied 8–10 min later. Representative traces of repeated experiments (n = 4).