Table 1.

Polymerase chain reaction primers

		Sequence (5′–3′)	Product size (bp)	Annealing temperature (°C)	Reference
GAPDH	Sense	ATCACCATCTTCCAGGAGCG	490	58.5	Law et al. (1999)
	Antisense	TAGGAACACGGAAGGCCATG
α-Smooth-muscle actin	Sense	GATCACCATCGGGAATGAACGC	389	57.5	Park et al. (1997)
	Antisense	CTTAGAAGCATTTGCGGTGGAC
SM-22	Sense	TGTTCCAGACTGTTGACCTC	369	54.9	Tsai & McKay (2000)
	Antisense	GTGATACCTCAAAGCTGTCC
Basic calponin	Sense	ACAAAAGGAAACAAAGTCAAT	396	55.1	GenBank D14437
	Antisense	GGGCAGCCCATACACCGTCAT
SM-1/SM-2 myosin heavy chains	Sense	AAGCAGCTCAAGAGGCAG	178 (SM-1)	57	Low et al. (1999)
	Antisense	AAGGAACAAATGAAGCCTCGTT	217 (SM-2)
Nestin	Sense	GGAGTGTCGCTTAGAGGTGC	262	57.3	Law et al. (1999)
	Antisense	CAGCAGAGTCCTGTATGTAGCC
Muscarinic m1 receptor	Sense	GCACAGGCACCCACCAAGCAG	373	60.7	Wei et al. (1994)
	Antisense	AGAGCAGCAGCAGGCGGAACG
Muscarinic m2 receptor	Sense	CACGAAACCTCTGACCTACCC	686	56.5	Wei et al. (1994)
	Antisense	TCTGACCCGACGACCCAACTA
Muscarinic m3 receptor	Sense	GTCTGGCTTGGGTCATCTCCT	434	57.4	Wei et al. (1994)
	Antisense	GCTGCTGCTGTGGTCTTGGTC
Muscarinic m4 receptor	Sense	TGGGTCTTGGCCTTTGTGCTC	588	59.1	Wei et al. (1994)
	Antisense	TTCATTGCCTGTCTGCTTTGTTA
Muscarinic m5 receptor	Sense	CTGGTCTCCTTCATCCTCTGG	394	57.6	Wei et al. (1994)
	Antisense	CCTGGGTTGTCTTTCCTGTTG
Endothelin type A receptor	Sense	TTCGTCATGGTACCCTTCGA	546	54	Kimura et al. (1999)
	Antisense	GATACTCGTTCCATACATGG
Endothelin type B receptor	Sense	TTCACCTCAGCAGGATTCTG	475	54	Kimura et al. (1999)
	Antisense	AGGTGTGGAAAGTTAGAACG
L-type Ca2+ channel	Sense	CAGAGCTGCCTCTTCAAAATCGC	437	57	Zeng et al. (1999)
	Antisense	ATTCAGGGCAATCTTCCCAAATACC
N-type Ca2+ channel	Sense	AAGAATGTCTTGAACATCCTGATCG	671	57	Zeng et al. (1999)
	Antisense	CAGCATCAGCTCGTACTCATAAGG