Figure 3. Effect of deprivation of compatible osmolytes on cell caspase-3 activity during hypertonic incubation.
Cells were incubated for the times indicated in depleted hypertonic (0.5 osmol (kg H2O)−1) medium with or without added betaine and myo-inositol, each at 0.1 mmol l−1. Caspase-3 activity was measured in cell extracts as described in the Methods section. Mean values (±s.d.) of three independent determinations are given. □, depleted of compatible osmolytes; ▪, with betaine and myo-inositol.