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Composition of solutions (mm)
| Relaxing | Activating | Dissecting | |
|---|---|---|---|
| Na2ATP | 5 | 5 | 5 |
| EGTA | 5 | — | 5 |
| CaEGTA | — | 5 | — |
| MgCl2 | 7 | 7 | 7 |
| Creatine phosphate | 22 | 20 | — |
| CPK (mg ml−1) | 1 | 1 | — |
| Cacodilic acid | 100 | 100 | — |
| Imidazole | — | — | 20 |
| KCl | — | — | 110 |
The ionic strength of the relaxing and activating solutions was 200 mm. pH of all solutions was adjusted to 7.0 with KOH and HCl. CPK, creatine phosphokinase. All chemicals were from Sigma.
