Table 1.
Leukocyte flow and microcirculatory data in postsinusoidal venules
| Sham | 1 h I–R | 1 h I–R + CrMP | 1.5 h I–R | 1.5 h I–R + CrMP | 1.5 h I–R + haemin | |
|---|---|---|---|---|---|---|
| Number of leukocytes analysed | 81 | 311 | 372 | 366 | 386 | 403 |
| Rolling leukocyte velocity (μm s−1) | 188.8 ± 11.7 | 165.6 ± 4.4 | 146.7 ± 6.1* | 131.9 ± 3.5† | 121.2 ± 3.7 | 183.8 ± 3.6‡ |
| Centreline RBC velocity (μm s−1) | 819.7 ± 32.7 | 634.8 ± 25.1* | 537.2 ± 20* | 593.8 ± 32.1* | 449.1 ± 18.1*†‡ | 569.8 ± 12.8* |
| Diameter of vessel (μm) | 50.8 ± 4.7 | 52.3 ± 2.4 | 48.9 ± 2.1 | 47.1 ± 2.8 | 45.1 ± 1.9 | 51.6 ± 2.3 |
| Wall shear rate (l s−1) | 84.9 ± 4.3 | 62.8 ± 4.7* | 55.7 ± 1.8* | 65.8 ± 1.9* | 48.2 ± 3.4*†‡ | 62.9 ± 3.1* |
Mean velocity (μm s−1) of rolling leukocytes was significantly lower than sham following 1.5 h. Inhibition of HO activity significantly reduced while induction of HO activity restored mean velocity of rolling leukocytes. Microhaemodynamics showed decreased centreline velocities in I–R-treated animals compared to sham animals. Inhibition of HO activity with CrMP in animals treated with 1.5 h of I–R caused a significantly lower centreline velocity and wall shear rate compared to only I–R-treated animals.
*
P < 0.05 compared to sham;
†
P < 0.05 compared to 1 h I–R;
‡
P < 0.05 compared to 1.5 h I–R.