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Expression of PAR-4. Southern blot analysis of PCRs on cDNA samples from RNA of HASMC, gel-filtered platelets, or CHRF-288-11 cells with RT. Control PCRs were done in parallel on each untreated RNA sample (−RT). The primers for the PCRs, the products of which were Southern blotted and probed with the appropriate nested primer probe, corresponded to PAR-4 and the positive control β-actin.
