Figure 4. Evidence for co-release of GABA and glycine from the stimulated pathway.

In the presence of external calcium (Ca²⁺) and flunitrazepam, a 200 pA IPSC exhibiting fast and slow decaying components was evoked by vestibular stimulation (holding potential −80 mV). When Ca²⁺ was replaced by strontium (Sr²⁺, 4 mm), the synchronous IPSC amplitude decreased (38 ± 6 %, n = 5) and asynchronous mIPSCs were observed. Among the asynchronous mIPSCs, dual component events were observed (arrows).