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. 2002 May 15;541(Pt 1):261–271. doi: 10.1113/jphysiol.2002.016832

Figure 5. Study B: effect of reduced pre-exercise muscle glycogen on mRNA content of metabolic genes in reponse to 3 h of two-legged dynamic knee-extensor exercise.

Figure 5

Summary data (means ± s.e.m.) from the control (▪) and low glycogen (□) trials showing fold change in mRNA content of the pyruvate dehydrogenase kinase 4 (PDK4), uncoupling protein 3 (UCP3), hexokinase II (HKII) and lipoprotein lipase (LPL) genes (relative to β-actin) in response to 3 h of two-legged knee-extensor exercise. Pre-exercise muscle glycogen content was manipulated by glycogen depletion exercise the previous day (both trials) followed by either a normal (control trial) or low (low glycogen trial) carbohydrate diet overnight. Total RNA was isolated from muscle biopsies obtained before exercise (Pre), after 1.5 and 3 h of exercise, and after 2 h of recovery. Significant (P < 0.05) main effects of glycogen (control vs. low) were found for PDK4 and UCP3, and of time (exercise and recovery) for PDK4, UCP3 and LPL. * Significantly (P < 0.05) different from Pre in the same trial. † Significantly (P < 0.05) different from the corresponding time point in the control trial.