Table 1.

Primers and reaction conditions used for PCR

Gene	Forward primer	Reverse primer	AT(°C)	[MgCl2](mM)	Size(bp)
UCP3	5′AGAACCATCGCCAGGGAGGAAGGA3′	5′CACCGGGGAGGCCACCACTGT3′	52	2.0	216
PDK4	5′CGGCTTGCCAATTTCTCGTCTGTA3′	5′TGATCCCGTAAAGTGTCCTGAGTG3′	53	2.0	272
HKII	5′TCAACCCCGGCAAGCAGAGG3′	5′CCGCCGGGCCACCACAGT3′	58	1.5	287
LPL	5′GTCCGCGGGCTACACCAAACT3′	5′ACTCGGGGCTTCTGCATACTCAAA3′	57	1.5	220
BA	5′CCCAAGGCCAACCGCGAGAAGAT3′	5′GTCCCGGCCAGCCAGGTCCAG3′	61	1.5	219

AT, annealing temperature; UCP3, uncoupling protein 3; PDK4, pyruvate dehydrogenase kinase 4; HKII, hexokinase II; LPL, lipoprotein lipase; BA, β-actin.