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. 2002 Jun 1;541(Pt 2):529–543. doi: 10.1113/jphysiol.2001.013438

Figure 2. Analysis of NHE protein expression in A6/C1 cells transfected with wild-type NHE3: typical immunoblot.

Figure 2

Membrane proteins (30 μg) of A6/C1 transfectants were resolved by SDS-PAGE, and probed for NHE1 or NHE3 antigen using polyclonal antibodies raised against the C-terminal 157 amino acids of the human NHE1 isoform (A) or against amino acids 822-835 of the rat NHE3 isoform (B). The expected mobility of the NHE proteins are indicated by the arrow. Each of the figures is representative of three independent experiments.