Figure 7. Analysis of protein expression from A6/C1 cells expressing wild-type and mutant NHE3s.

The level of protein expression of wild type (WT) and mutant NHE3 was determined by immunoblot. 30 μg of membrane protein from each cell line was resolved by SDS-PAGE, transferred onto nitrocellulose filters and probed with antibodies specific for the C-terminal domain of the human NHE3 isoform using ECL. Abbreviations in the figure are used to refer to the various NHE3 constructs transfected into A6/C1 cells and are those given in Table 1. Two independent experiments showed similar results.