. 2002 Jun 1;541(Pt 2):367–383. doi: 10.1113/jphysiol.2001.016022

Table 2.

Desensitization kinetics of cell-attached patches (1 mM ACh)

Five-component patches, Linear model
	k₀₁	k₁₀	k₁₂	k₂₁	k₂₃	k₃₂	k₃₄	k₄₃	k₄₅	k₅₄^*	est. N
	46.7	644.8	844.2	113.1	56.2	11.3	7.32	0.75	0.15	0.20	53
	65.7	778.9	1727.0	335.0	166.0	38.9	18.70	1.17	0.71	0.31	83
	67.6	969.3	303.9	61.7	48.9	6.9	1.55	0.50	0.12	0.08	21
	133.0	451.2	671.6	178.0	91.7	32.5	8.30	2.39	0.33	0.42	112
	43.8	522.8	421.5	86.5	46.7	27.6	11.60	1.28	0.59	0.23	61
Mean	71.4	673.4	793.6	154.9	81.9	23.4	9.49	1.22	0.38	0.25	66
s.e.m.	16.1	92.6	251.7	49.0	22.5	6.2	2.81	0.33	0.18	0.13	15

Five-component patches, Star model
	k₀₁	k₁₀	k₀₂	k₂₀	k₀₃	k₃₀	k₀₄	k₄₀	k₀₅	k₅₀^*
	18.0	1601	12.4	106.6	7.3	12.2	4.09	0.55	4.99	0.11
	16.6	2768	17.4	272.8	15.7	31.0	5.32	1.40	10.73	0.11
	48.1	1389	6.4	277.0	5.7	51.6	5.98	3.16	1.99	0.20
	43.0	1246	35.8	167.9	35.3	20.7	15.10	1.84	4.57	0.32
	22.1	988	8.3	108.5	8.7	22.0	2.30	1.37	2.45	0.11
Mean	30	1598	16	187	15	28	6.5	1.7	4.9	0.15^*
s.e.m.	7	308	5	38	6	7	2.2	0.4	1.6	0.04

Four component patches, Linear model
	k₀₁	k₁₀	k₁₂	k₂₁	k₂₃	k₃₂	k₃₄	k₄₃^*
	38.6	302	692	65.0	52.0	2.3	0.5	0.80
	68.6	433	418	107.0	130.0	18.1	11.5	0.64
	60.9	334	565	65.6	45.2	8.8	13.8	1.46
	18.6	394	367	54.5	18.6	1.9	1.7	0.08
	34.0	587	708	169.0	61.9	14.0	6.9	0.31
Mean	44	410	549	92	62	9.0	6.8	0.66
s.e.m.	9	49	69	21	19	3.2	2.6	0.23

Four component patches, Star model
	k₀₁	k₁₀	k₀₂	k₂₀	k₀₃	k₃₀	k₀₄	k₀₃^*
	13.2	1398.3	11.4	134.3	5.5	13.7	4.0	0.2
	29.4	919.5	13.6	186.3	8.9	16.1	16.6	0.2
	20.5	946.0	12.4	74.3	7.0	16.4	21.1	0.3
	8.9	789.5	5.9	46.1	1.6	2.9	2.2	0.0
	13.2	1398.3	11.4	134.3	5.5	13.7	4.0	0.2
Mean	17	1090	11	115	5.7	13	9.5	0.16
s.e.m.	4	128	1	25	1.2	2	3.9	0.03

The kinetic models are shown in Fig. 12 (rate constants, s⁻¹).

The slowest recovery rate constant is predicted to scale linearly with the number of channels in the patch. The number of components refers to the shut interval duration distribution. The estimated number of channels (est. N) in the five-component patches is the ratio of k₅₄ * and the single-channel value of for this rate constant (see Table 4). The number of intervals in each patch is shown in Table 1.