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. 2002 Jun 1;541(Pt 2):351–366. doi: 10.1113/jphysiol.2002.017749

Figure 6. 8-Br-cGMP mimics the effects of SNP by reducing L-channel activity through a PKG-mediated mechanism.

Figure 6

A, cell exposure to 400 μM 8-Br-cGMP (right) reduces the probability of channel opening with respect to controls (left). B, after incubation with the specific PKG inhibitor, KT-5823 (1 μM), 8-Br-cGMP fails to evoke the marked reduction in NPo shown in A. Bottom traces in A and B are averaged currents obtained from 10 (control) and 40 traces (drug) in both cases. C and D show the mean NPo values versus time obtained from 5 patches. Filled bars in C and D are mean control values collected over 1 min; open bars are mean values obtained by averaging data over 30 s periods during application of 8-Br-cGMP and 8-Br-cGMP + KT-5823, respectively. The inset in C shows normalized mean NPo with respect to controls exposed to KT-5823 for about 10 min (n = 4), pretreated with KT-5823 and then exposed to 8-Br-cGMP (n = 5), or exposed to 8-Br-cGMP alone (n = 5). All the patches contained multichannel openings.