Table 1.
Rat small-intestinal surfactant-like particle (SLP) binding to extracellular matrix (ECM) proteins
| ECM protein | SLP added (ng) | SLP bound | |
|---|---|---|---|
| (ng ± s.d.) | (%) | ||
| Collagen type I | 75 | n.d. | 0 |
| 750 | 142 ± 28 | 19 | |
| Collagen type IV | 75 | n.d. | 0 |
| 880 | 54 ± 13 | 6 | |
| Laminin | 90 | 87 ± 33 | 89 |
| 75 | 128 ± 3 | 17 | |
| Fibronectin | 75 | 71 ± 18 | 95 |
| 600 | 93 ± 10 | 16 | |
Wells were coated with 4-5 μg of ECM protein, air dried, blocked with with 4% bovine serum albumin, and dried again before adding rat small-bowel SLP. An antibody to SLP was added, followed by a biotinylated goat anti-rabbit IgG and streptavidin linked with horseradish peroxidase. The presence of antibody was revealed using 3,3′-diaminobenzidine and H2O2 at room temperature for 3–5 min. The amount of SLP bound was red from a standard curve using purified SLP. N = 4; n.d. = not detected.