Figure 4.

Pattern of expression of Kv4.1 in the CB, as revealed by in situ hybridization in cultured cells, and colocalization with other Kv subunits

A and B, control experiments showing colocalization of the TH transcript, as revealed by in situ hybridization (A), and protein, as revealed by immunofluorescence (B), in chemoreceptor cells (arrows). C, in situ hybridization of cultured CB cells with Kv4.1 riboprobe. Arrows indicate Kv4.1-positive cells. D-F, colocalization of the Kv4.1 transcript (D), TH protein (E) and the Kv4.3 subunit (F) in CB chemoreceptor cells (examples indicated by arrows). A number of Kv4.1-positive cells are not chemoreceptor cells (arrowheads). G-I, colocalization of Kv4.1 mRNA in a population of CB cells expressing Kv3.4. Chemoreceptor cells (indicated by arrows) expressing Kv4.1 (G) and TH (H), also exhibit Kv3.4 labelling (I). A population of chemoreceptor cells express Kv4.1 (arrowheads in G and H), but do not express Kv3.4 (arrowheads in I).