Figure 10. Effects of H-89 (A) or NPY after H-89 treatment (B).

Data represent percentage changes in the number of transported particles. Cells were treated with the protein kinase A inhibitor H-89 (1 μM) alone (A), or NPY (1 μM) after treatment with H-89 (1 μM; B). Note that H-89 inhibited anterograde and retrograde axonal transport, but the subsequently applied NPY failed to produce a further decrease in axonal transport. Each data point indicates the mean (± s.d.) of the values obtained from five DRG cells. * P < 0.05, ** P < 0.005, *** P < 0.0005 compared to the value before the application of H-89.