Figure 7. Radioactive outflow from SCG cultures loaded with [³H]-NA.

A, fractional rate of the release of radioactivity in response to 0.3 μM reserpine (○, application indicated by open bar), and in response to 0.3 μM reserpine followed by 0.5 μM desipramine (•, application indicated by filled bar). Data points are means ± s.e.m. from 3 individual cultures included in the same release experiment. B, fractional rate of radioactive outflow in response to 2.0 μM cyanine 863 (application indicated by bar). Data points are means ± s.e.m. from 3 individual cultures included in the same release experiment. C, AUC values of radioactive outflow induced by indicated test substances (number of individual cultures in parenthesis). Des: 0.5 μM desipramine; Res: 0.3 μM reserpine; Cy 0.5, 2.0, 10: cyanine 863 (μM); d-TC 50, 100, 250: d-tubocurarine (μM); Estr 10, 30: oestradiol (μM); Amant: 50 μM amantadine; Guan: 10 mm guanidine; TEA: 10 mm tetraethylammonium Cl⁻. Bars indicate means ± s.e.m.. All data points differ significantly from zero (P < 0.01, Mann-Whitney U test).