Figure 8. Radioactive metabolites in supernatants of cultures loaded with [³H]-NA.

SCG cultures were loaded with [³H]-NA and rinsed for 60 min as described in Methods. Cultures were then incubated for 30 min, with either 0.3 μM reserpine (A) or 2.0 μM cyanine 863 (B) and with 0.5 μM desipramine (Des), 0.5 μM clorgyline (Clor) and 1 μM OR 486 to inhibit the NAT, MAO_A and COMT, respectively. Thereafter, the radioactivity in supernatants was analysed for the presence of DOMA (dl-3,4-dihydroxymandelic acid), DHPG (dl-3, 4-dihydroxyphenyl glycol), VMA (3-methoxy-4-hydroxymandelic acid), MHPG (3-methoxy-4-hydroxyphenylglycol), NA, adrenaline and NMN (normetanephrine). Box plots indicate the relative proportion that each of the 3 major compounds ([³H]-NA, [³H]-DHPG and [³H]-NMN) contributes to the total radioactivity recovered from supernatants (expressed as %). Figures in parentheses show the number of supernatants that have been analysed for indicated experimental approaches.