Figure 6. SNP- and DBcGMP-induced release of TXB₂ into the bathing solution from mucosa set between Ussing chambers.

The mucosa was incubated for 35 min in the absence of SNP and DBcGMP, then the TXB₂ concentration in the serosal solution was determined (open columns). Fresh Parsons solution was introduced and the mucosa was incubated successively for 15 min in the absence of SNP and DBcGMP, and for 20 min in the presence of 1–100 μM SNP (A) or 500 μM DBcGMP (B). Then the TXB₂ concentration in the serosal solution was determined (filled columns). n = 4. *,** P < 0.05 and 0.01, respectively. NS, P > 0.05.