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A, typical presynaptic Ca2+ transients under control conditions and after pre-incubation with 1 and 5 μM EGTA-AM in addition to OGB-1. Extracellular Ca2+ concentration: 5 mm. B, effect of EGTA-AM on the peak amplitude (▵) and decay time constants (τ, ▴) of presynaptic Ca2+ transients. C, paired pulse ratios of eIPSCs at inter-stimulus intervals > 500 ms were not affected by EGTA and CLZ.
