Figure 4. Effect of sarcoplasmic reticulum Ca²⁺ store depletion on the cytosolic [Ca²⁺] in pulmonary and renal ASMCs voltage clamped at -40 mV.

A, 10 mm CAF and 1 μM ANG II-induced [Ca²⁺] (continuous line) and net membrane current (dotted line) responses in the presence of 10 μM RY and 10 μM CPA in a single pulmonary ASMC. The dashed line shows the resting cytosolic [Ca²⁺]. B, change in the cytosolic [Ca²⁺] from resting levels in pulmonary (▪) and renal (□) ASMCs. Error bars represent ± s.e.m. Numbers of independent experiments are shown in parentheses. * Mean value significantly different from control; paired two-tailed t test.