Table 1.

Effect of indomethacin on alkali secretion

	Control	Indomethacin	Indo + dm-PGE2
Untreated	12.7 ± 2.8	2.2 ± 1.7*	21.8 ± 2.7
+ Omeprazole	29.4 ± 4.5	13.0 ± 4.0*	20.1 ± 3.4

Results are given as nmol OH⁻¹ min⁻¹. Whole stomachs of anaesthetized mice were perfused with a weakly buffered saline solution of pH 3 at 0.2 ml min⁻¹. Tissues were either untreated or exposed to 60 mg kg⁻¹ omeprazole I.P. for 60 min. The perfusion speed was slowed to 0.1ml min⁻¹ and the pH change and buffering capacity of the solution used to determine the net amount of alkali added to the gastric effluent (see Appendix). Indomethacin (5 mg kg⁻¹) was then added I.P., and after 60 min slow flow was again used to measure net alkali secretion. Finally, 0.1mg kg⁻¹ dm-PGE₂ was added I.P. and slow flow was used to measure net alkali secretion in the following 10 min.

Means ± s.e.m., n = 4

^*P < 0.05versus control.)