Skip to main content
. 2002 Sep 6;544(Pt 3):883–896. doi: 10.1113/jphysiol.2002.021972

Figure 2. Reproducibility of vasodilatation by successive applications of ACh and its inhibition by NOS blockade.

Figure 2

Variations in relative vascular conductance (RVC; mean ± s.e.m.) as a function of time in three experimental series. A, control response to successive ACh applications. ACh (100 μm) was applied topically for 10 min periods in the superfusion medium at indicated times (bars). B, time course of NOS inhibition and effect of SNP. After a 30 min control period, the cheek pouch was superfused with 10 μml-NNA for the remainder of the experiment (arrow). After 2 h of NOS inhibition, 10 μm SNP was applied topically (bar). The dotted line depicts the initial basal RVC level. C, time dependence of l-NNA treatment on ACh-induced vasodilatation. After 30 min in control conditions, the tissue was superfused continuously with 10 μml-NNA for 210 min. ACh (100 μm) was applied at 60 and 120 min after starting NOS inhibition.