Figure 3. Effect of diclofenac without and with charybdotoxin + apamin on bradykinin-induced hyperpolarization in an endothelium-intact strip from a normotensive pregnant woman.

A, original trace of the effect of bradykinin (0.1 μm) on membrane potential in smooth muscle cells of an endothelium-intact strip from a normotensive pregnant woman (Control). B, effect of the cyclooxygenase inhibitor, diclofenac (DF). After recording the control response (shown in A), the strip was pretreated with DF (3 μm) and bradykinin was applied in the presence of DF. C, effect of the Ca²⁺-activated K⁺ channel inhibitors, charybdotoxin (CTX) + apamin, in the presence of diclofenac. In the presence of diclofenac, the strip was pre-treated for 15 min with CTX (0.1 μm) + apamin (0.1 μm) and bradykinin was applied in the presence of DF + CTX + apamin.