Table 3.

Effect of diclofenac with and without CTX + apamin on bradykinin-induced hyperpolarization

	Normotensive pregnant (mV)	Pre-eclampsia (mV)
I. Resting membrane potential
Control	−59.9 ± 1.3 (9)	−56.0 ± 1.1 (6)
+DF	−55.1 ± 0.8†(9)	−53.2 ± 1.2 (6)
+DF + CTX + apamin	−51.8 ± 0.4‡(4)	−49.4 ± 0.7‡(4)
II. Bradykinin-induced hyperpolarization
Control
initial hyperpolarization	−12.4 ± 1.4 (6)	−11.3 ± 1.2 (6)
second hyperpolarization	−4.3 ± 0.7 (6)	−2.6 ± 0.4* (6)
+DF
initial hyperpolarization	−11.5 ± 1.8 (6)	−16.4 ± 1.0 (4)
second hyperpolarization	−0.4 ± 0.6†(6)	−0.4 ± 1.6 (4)
+DF + CTX + apamin
initial hyperpolarization	−0.6 ± 0.6‡(4)	−1.2 ± 1.2‡ (3)

Data are expressed as mean ± s.e.m. The number of women is given in parentheses. The initial hyperpolarization was calculated as the maximum hyperpolarization obtained within a 3 min period after application of bradykinin, and the second hyperpolarization was calculated as the maximum hyperpolarization obtained within a 5–17 min period after washout of bradykinin. The cyclooxygenase inhibitor, diclofenac (DF, 3 μm), was given as pretreatment for 60 min and was present throughout the experiment. Charybdotoxin (CTX) + apamin (Ca²⁺-activated K⁺ channel inhibitors) were given as pretreatment for 15 min in the presence of diclofenac and were present thereafter.

^*P < 0.05 vs. normotensive pregnant women.

^†P < 0.05 vs. before application of diclofenac.

^‡P < 0.05 vs. before application of CTX + apamin.