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. 2002 Oct 18;545(Pt 2):485–499. doi: 10.1113/jphysiol.2002.031161

Figure 4. Immunofluorescence staining of ZO-1 and F-actin.

Figure 4

Cells were analysed with ZO-1 antibody (green) and Alexa Fluor 594-phalloidin (red) for F-actin at 3 min (1-3) and 10 min (4-6) p.l. Images 1 and 4, ZO-1; 2 and 5, F-actin; 3 and 6 merged images of ZO-1 and F-actin staining. The arrangement of images (x-y, x-z, y-z planes) is the same as in Fig. 3. The scale bar (10 μm) applies to all images. (m: damage caused by marks indicating the position of the defect investigated. The mark consisted of lines in a right-angled pattern that were scratched into the monolayer with a microelectrode.)