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. 2002 Oct 18;545(Pt 2):485–499. doi: 10.1113/jphysiol.2002.031161

Figure 5. Conductivity of HT-29/B6 monolayers measured in a conventional Ussing chamber as a function of time.

Figure 5

A, the time course was recorded under control conditions (n = 19) and the effects of cytoskeleton modulators, cytochalasin D (n = 10) or butanedione monoxime (n = 10), and low free extracellular calcium (250 nmol l−1, n = 8) were studied. With both inhibitors and under low free calcium, the increase of GUssing was reversible upon washout with regular Ringer solution. B, cells from a different passage (32nd-33rd) were analysed under control conditions (n = 14) and the effects of the myosin light chain kinase inhibitor ML-7 (n = 7), and the Rho-associated kinase inhibitor Y-27632 (n = 6) were studied. With the inhibitor ML-7, the increase of GUssing was reversible upon washout with regular Ringer solution, but the effect of Y-27632 was not.