Figure 7. Conductance of the leak, g_leak, as a function of time associated with single-cell defects.

Time course of restitution in HT-29/B6 cells (28th-31st passage), under control conditions (A), after incubation with cytochalasin D (B), with 2,3-butanedione monoxime (C), and in low free [Ca²⁺]_o Ringer solution (D). Under control conditions (A), repair resulted in exponential decline of g_leak, which allowed extrapolation (dashed line) of the initial (t + 0) leak at 0.69 ± 0.06 μS. Sealing of the leak (described by g_leak) was blocked after inhibition of actin polymerization (B), and was slower after impairment of myosin ATPases (C), but proceeded at the same speed in low Ca²⁺ Ringer solution (D).