Figure 4. Current responses to different voltage steps determined after three distinct conditioning pulses in cells expressing Q552R hClC-1 channels.

Current responses of a single cell to a pulse protocol consisting of a fixed conditioning pulse and a variable pre-pulse (from −145 mV to +95 mV in 40 mV intervals), followed by a fixed step to −125 mV. The following conditioning potentials were applied: 0 mV (A), −125 mV (B) and +125 mV (C).