Figure 5. Effects of −20 mV HP (E_NCX at a 3:1 stoichiometry) and −90 mV HPs on E_NCX during the recovery of I_NCX from Ni²⁺ inhibition.

A, concatenated currents at −20 mm HP which is E_NCX at a 3:1 stoichiometry. B, concatenated currents at −90 mV HP which is a more negative voltage than E_NCX at a 3:1 stoichiometry. C, difference I-V curves of the corresponding labels from A. E_NCX was at about −20 mV and did not shift with time. D, difference I-V curves of the corresponding labels from B. E_NCX developed initially at around −20 mV and then shifted toward −34 mV with time.