Table 1.
Quantification of XOR colocalization with adipophillin (ADPH) and butyrophilin (Btn) at the apical plasma membrane
| Apical membrane area; mean ± s.e.m.(μm2) | Percentage overlap | |
|---|---|---|
| XOR and ADPH colocalization | ||
| XOR | 4.40 ± 0.44 | 73% |
| ADPH | 4.65 ± 0.47 | 68% |
| Overlap | 3.10 ± 0.27 | — |
| XOR and Btn colocalization | ||
| XOR | 5.86 ± 0.85 | 76% |
| Btn | 11.08 ± 2.0 | 50% |
| Overlap | 4.39 ± 0.51 | — |
The overlap between XOR and ADPH immunofluorscence and XOR and Btn immunofluorescence at the apical plasma membrane of alveolar epithelial cell was quantified in mammary glands at day 10 of laction. The results are shown as the average (± s.e.m.) statining are (μm2) per field for each proteing, the area of staining overlap and the percent overlap (overlap area/total apical membrane staining area of each protein – 100). Areas were calculated using the Masking Algrothm of Slidebook and represent averages of 15separate fields from two animals.