Table 2.
Expressed proteins | Observed Tdeact (S) | Expected Mean Tact (S) | Observed Tact (S) | Expressed amplituted (% of control) | Observed amplituted (% of control) |
---|---|---|---|---|---|
Gαi1* | 17.6 ± 4.6 | 11.5 | 11.2 ± 3.8 | — | — |
Gαi1*+ RGS4 | 8.1 ± 5.0 | 6.5 | 5.3 ± 2.2 | 56 ± 28 | 36 ± 5 |
Gαi1*+ RGS7 | 12.5 ± 6.1 | 9.1 | 8.8 ± 2.9 | 79 ± 25 | 74 ± 6 |
Gαi1*+ RGS7 + Gβ1 | 17.5 ± 2.9 | 11.5 | 13.0 ± 2.6 | — | — |
Gαi1*+ RGS7 + Gβ5 | 13.9 ± 2.1 | 9.8 | 11.4 ± 2.5 | 85 ± 9 | 88 ± 17 |
Gαi2* | 23.8 ± 7.1 | 13.9 | 15.7 ± 7.1 | — | — |
Gαi2*+ RGS4 | 9.6 ± 6.3 | 7.5 | 6.1 ± 2.1 | 54 ± 23 | 33 ± 6 |
Gαi2*+ RGS7 | 17.1 ± 6.4 | 11.3 | 10.8 ± 4.3 | 81 ± 18 | 90 ± 8 |
Gαi2*+ RGS7 + Gβ1 | 20.3 ± 3.6 | 12.6 | 14.8 ± 2.9 | — | — |
Gαi2*+ RGS7 + Gβ5 | 18.6 ± 2.9 | 11.9 | 15.7 ± 7.5 | 95 ± 9 | 100 ± 18 |
Gαi3* | 15.4 ± 3.4 | 10.5 | 14.1 ± 5.2 | — | — |
Gαi3*+ RGS4 | 10.0 ± 5.3 | 7.7 | 5.7 ± 2.0 | 73 ± 27 | 37 ± 3 |
Gαi3*+ RGS7 | 13.5 ± 4.4 | 9.6 | 10.5 ± 3.6 | 91 ± 20 | 61 ± 4 |
GαoA* | 16.7 ± 2.8 | 11.1 | 6.8 ± 4.1 | — | — |
GαoA*+ RGS4 | 5.7 ± 1.9 | 4.9 | 4.2 ± 1.9 | 44 ± 12 | 105 ± 11† |
GαoA*+ RGS7 | 3.3 ± 1.7 | 3.0 | 3.1 ± 1.8 | 27 ± 12 | 79 ± 8† |
GαoA*+ RGS7 + Gβ1 | 6.3 ± 2.2 | 5.3 | 2.9 ± 1.5 | — | — |
GαoA*+ RGS7 + Gβ5 | 2.2 ± 1.3 | 2.0 | 0.9 ± 0.5 | 39 ± 21 | 17 ± 2 |
Gαob* | 14.8 ± 4.9 | 10.3 | 6.0 ± 3.4 | — | — |
Gαob*+ RGS4 | 6.1 ± 2.9 | 5.2 | 5.0 ± 1.4 | 50 ± 19 | 105 ± 9† |
Gαob*+ RGS7 | 4.0 ± 1.5 | 3.6 | 3.1 ± 0.7 | 35 ± 11 | 91 ± 13† |
Gαob*+ RGS7 + Gβ1 | 2.7 ± 1.0 | 2.5 | 2.4 ± 0.7 | — | — |
Gαob*+ RGS7 + Gβ5 | 1.4 ± 0.9 | 1.3 | 1.7 ± 1.0 | 54 ± 32 | 32 ± 3 |
denotes deviation from expected steady—state GIRK current amplitude.