Figure 1. Manual and algorithmic identification of sPSCs, choice of the template and recording mode.

A, the first 2 s of a recording epoch broken up into 200 ms periods, each vertically separated. The total number of events detected by eye was 67, of which 65 were recovered (•) and two were considered as missed (○) by the template-matching algorithm. B, the template was chosen from a representative PSC. The template is truncated (continuous line), with the truncated portion of the time course indicated by the dashed line. C and D, two subsequent recording sequences from the same cell illustrated in voltage- and current-clamp, respectively. E, the two templates used to detect spontaneous synaptic currents in voltage- (black) and current-clamp (grey). The current-clamp template has been inverted to allow for better comparison. F, cumulative probability density functions (cPDF) of f₁ for the data recovered in voltage- and current-clamp shown in black and grey, respectively.