Figure 5. Pharmacological profile of mEPSCs.

A, control recording epoch and B, after the addition of bicuculline. The cPDFs of f₁ (C) and amplitudes (D) before (black line) and after the block of inhibition (grey line) with the average time courses shown in the inset. mEPSCs are mediated by AMPA receptors; E, recording epoch in control ACSF and F, after the addition of 10 μm NBQX. Pharmacological measures to reduce the frequency (G) and amplitude (H) of mEPSCs show that only AMPA receptor blockade has an effect on mPSC frequency. Data are pooled after normalising to control condition. Asterisks indicate significant changes. The abbreviations indicate ACSF to which tetrodotoxin (TTX, n = 18), bicuculline (Bic, n = 10), kynurenic acid (Kyn, n = 6), NBQX (n = 3), glutamine (Glut, n = 5), Mg²⁺ (n = 5), Cd²⁺ (n = 7), Ni²⁺ (n = 5) or APV (n = 6) was added. LES indicates low excitability solution (n = 9).