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. 2002 Nov 1;545(Pt 3):829–836. doi: 10.1113/jphysiol.2002.029843

Figure 2. Immunological analysis of BKCaα-subunit expression in SMCs and ECs from bovine coronary arteries.

Figure 2

A, Western immunoblot analysis showed an immunoreactive band corresponding to the BKCaα-subunit (125 kDa) in the three left lanes loaded with SMC membrane protein, but not in the three right lanes loaded with EC membrane protein. B, the immunoreactive bands in SMC protein detected in two left control lanes (-CP) were not evident when the antibody directed against the α-subunit was preabsorbed with its antigenic competing peptide in the two right lanes (+CP). C, Nomarski and corresponding fluorescent images of SMCs and ECs labelled for the BKCaα-subunit (red). Nuclei were labelled with DAPI (blue). Only SMCs showed positive labelling for the BKCaα-subunit. D, the positive labelling for the BKCaα-subunit in SMCs was not present when the antibody was preabsorbed with its antigenic competing peptide (+CP).