. 2002 Nov 1;545(Pt 3):961–974. doi: 10.1113/jphysiol.2002.031484

Table 3.

The effects of ischaemic and diazoxide preconditioning with and without 5-hydroxydecanoate on mitochondrial citrate synthase activity, matrix volume and ADP-stimulate substrate oxidation

	n	Citrate synthase (units (mg protein)⁻¹)	Matrix volume (μl(mg protein)⁻¹)	Rate of State 3 substrate oxidation (nmol O min⁻¹ (mg protein)⁻¹)			Rate of State 3 substrate oxidation (Ratio to ascorbate + TMPD)

				2-Oxoglutarate	Succinate	Ascorbate + TMPD	2-Oxoglutarate	Succinate
Preischaemia
Control	8	3.02 ± 0.11	0.65 ± 0.05	371 ± 29	357 ± 32	1173 ± 164	0.34 ± 0.03	0.32 ± 0.03
5HD (100 μm)	6	2.36 ± 0.10^*	1.15 ± 0.09^*	323 ± 28	281 ± 14^*	1259 ± 132	0.28 ± 0.05	0.24 ± 0.03
5HD (300 μm)	6	2.50 ± 0.08^*	1.23 ± 0.05^*	311 ± 12	279 ± 21	1264 ± 159	0.27 ± 0.04	0.23 ± 0.02
IPC	8	2.68 ± 0.16^*	0.87 ± 0.08^*	474 ± 36^*	489 ± 33^*	1183 ± 78	0.42 ± 0.03	0.42 ± 0.04
IPC + 5HD (100 μm)	6	2.32 ± 0.10^*	1.07 ± 0.05^*^§	398 ± 36	396 ± 27^§	1138 ± 150	0.40 ± 0.09	0.38 ± 0.06
IPC + 5HD (300 μm)	6	2.2 ± 0.13^*	1.19 ± 0.07^*^§	408 ± 22	359 ± 16^§	989 ± 171	0.47 ± 0.09	0.44 ± 0.09
Diazoxide (50 μm)	6	2.44 ± 0.11^*	1.03 ± 0.07^*	291 ± 21^*	306 ± 34	1345 ± 243	0.26 ± 0.05	0.26 ± 0.04
Diazoxide + 5HD (100 μm)	6	2.47 ± 0.10^*	1.15 ± 0.07^*	293 ± 22^*	287 ± 15	1092 ± 118	0.29 ± 0.05	0.28 ± 0.04
End-Ischaemia
Control	6	2.24 ± 0.13^†	0.91 ± 0.08^†	191 ± 18^†	222 ± 20	479 ± 34^†	0.43 ± 0.06	0.47 ± 0.07
5HD (100 μm)	6	1.99 ± 0.10	1.36 ± 0.14^*	1.66 ± 20^†	194 ± 23^†	530 ± 55^†	0.33 ± 0.02	0.35 ± 0.03
5HD (300 μm)	6	2.19 ± 0.06^†	1.29 ± 0.15^*	179 ± 22^†	200 ± 18^†	582 ± 47^†	0.33 ± 0.04	0.35 ± 0.05^†
IPC	6	2.26 ± 0.15	1.21 ± 0.05^*^†	235 ± 21^†	276 ± 24^†	523 ± 35^†	0.46 ± 0.06	0.54 ± 0.07
IPC + 5HD (100 μm)	6	2.38 ± 0.05	1.25 ± 0.08^*	213 ± 17^†	239 ± 22^†	725 ± 75^*^§^†	0.31 ± 0.03^§	0.34 ± 0.04^§
IPC + 5HD (300 μm)	6	2.40 ± 0.08	1.37 ± 0.11^*	211 ± 17^†	268 ± 13^†	736 ± 34^*^§	0.31 ± 0.04^§	0.36 ± 0.03^§
Diazoxide (50 μm)	6	2.14 ± 0.18	1.22 ± 0.11^*	183 ± 27^†	239 ± 20	743 ± 62^*^†	0.27 ± 0.05^*	0.32 ± 0.03^*
Diazoxide + 5HD (100 μm)	6	2.15 ± 0.10^†	1.19 ± 0.06	213 ± 25^†	215 ± 15^†	657 ± 72^*^†	0.37 ± 0.05	0.33 ± 0.05^*
Reperfusion
Control	7	1.97 ± 0.30^†	0.87 ± 0.07^†	408 ± 70^‡	402 ± 69^‡	1034 ± 77^‡	0.43 ± 0.09	0.38 ± 0.07
5HD (100 μm)	6	1.95 ± 0.19	1.00 ± 0.05^‡	205 ± 16^*^†	240 ± 17	757 ± 117^*^‡	0.31 ± 0.05	0.35 ± 0.06
5HD (300 μm)	7	1.41 ± 0.13^†^‡	1.00 ± 0.07	238 ± 21^*^†^‡	235 ± 12^*	541 ± 58^*^†	0.50 ± 0.07^†^‡	0.43 ± 0.04^†
IPC	9	236 ± 0.34	0.90 ± 0.09^*^‡	403 ± 84	387 ± 72	1094 ± 73^‡	0.38 ± 0.08	0.36 ± 0.07^‡
IPC + 5HD (100 μm)	8	2.05 ± 0.26	1.17 ± 0.09^*	326 ± 25^‡	323 ± 27^‡	976 ± 117	0.40 ± 0.08	0.39 ± 0.08
IPC + 5HD (300 μm)	8	1.41 ± 0.10^†^‡^§	1.03 ± 0.06^‡	460 ± 37^‡	453 ± 27^†^‡	714 ± 32^*^§^†	0.67 ± 0.04^*^§^†^‡	0.62 ± 0.04^*^§^†^‡
Diazoxide (50 μm)	7	2.30 ± 0.07	1.01 ± 0.08	310 ± 27^‡	296 ± 25	881 ± 51^†	0.37 ± 0.02	0.33 ± 0.02
Diazoxide + 5HD (100 μm)	5	2.46 ± 0.08^‡	0.92 ± 0.10^†^‡	316 ± 27^‡	279 ± 27^‡	1021 ± 106^‡	0.32 ± 0.02	0.27 ± 0.01

The rates of ADP-stimulated (State 3) oxidation of 2-oxoglutarate + malate and succinate are expressed relative to that of ascorbate TMPD to correct for changes in respiratory chain activity that are independent of changes in matrix volume. The mitochondrial protein contents was determined from the citrate synthase activity, since this eliminates errors caused by the presence of variable amounts of broken mitochondria under the different conditions. All data are presented as means ±s.e.m. for the number of hearts shown, whose haemodynamic performance is summarized in Table 2

P < 0.05vs. control

^§

vs IPC

^†

vs pre-ischaemia

^‡

vs end-ischaemia