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. 2008 Mar 14;105(12):4939–4944. doi: 10.1073/pnas.0800211105

Fig. 2.

Fig. 2.

Altered mRNA levels of target genes and binding of CCA1 protein to target gene promoter regions validate predicted regulation by CCA1. (A) RT-qPCR was performed on CCA1-ox, glk1 knockout, and wild-type plants to determine mRNA levels for ASN1, GLN1.3, and GDH1. Three biological and two technical replicates were carried out for each sample. mRNA levels were normalized to clathrin (At4g24550). The mean ± standard error of the mean is shown. (B) ChIP assays to show binding of CCA1 to GLN1.3, GDH1, bZIP1, TOC1, and ZTL gene promoter regions. Control, input DNA control (no IP); −Ab, IP without antibody; CCA1, IP with the CCA1 antibody.