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. 2008 Mar 13;105(12):4808–4813. doi: 10.1073/pnas.0710907105

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© 2008 by The National Academy of Sciences of the USA

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Fig. 1. — Type I IFN-dependent phosphorylation/activation of Akt. (A and B) Akt1^+/+2^+/+, Akt1^−/−2^+/+, and Akt1^−/−2^−/− MEFs were treated with mouse IFN-α for the indicated times. Equal protein aliquots were processed for immunoblotting with antibodies against phosphorylated forms of Akt on Ser-473 (A) or Thr-308 (B). Respective blots were stripped and reprobed with a pan anti-Akt antibody as indicated. (C) Akt1^+/+2^+/+ and Akt1^−/−2^−/− MEFs were treated with mouse IFN-β for the indicated times. (Top) Equal protein aliquots were immunoblotted with an antibody against the phosphorylated form of Akt on Ser-473. (Middle) Equal protein aliquots from the same experiment were resolved on separate gels and probed with an anti-Akt antibody. (Bottom) The blot shown in Top was reprobed with an anti-tubulin antibody to control for protein loading.