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. 2008 Mar 17;105(12):4745–4750. doi: 10.1073/pnas.0705680105

Fig. 6.

Fig. 6.

Chronic hypoxia-induced cell death is independent of the apoptotic and/or autophagic pathways. Cells were incubated in hypoxia (1% O2) for different periods of time. (a) Measurement by flow cytometry of the sub-G1 PI-positive population in CCL39 and GSK3 cells. (b) PARP and LC3 proteins were analyzed by Western blotting on GSK3 extracts. Staurosporin (1 μM) was used as an apoptotic cell death activator. Nutrient-starved cells were used as a positive control for autophagy.