Fig. 7.

Mice adapt to chronic hypoxia by activating PHDs to desensitize HIFα. (a) BL6 mice were exposed or not to hypoxia at 8%O₂ for 6 or 24 h or to 8%O₂+2h at 6%O₂ for 24 h. HIF-1α staining in kidney was analyzed by immunohistochemistry. (b) pVHL capture assay was performed with brain extracts from mice exposed to the experimental conditions described in a. (c) BL6 mice were injected for 7 days with siRNAs targeting the three PHD isoforms or an irrelevant sequence before being exposed to hypoxia at 8% O₂ for 24 h. Control mice were also injected with DFO (1,200 mg/kg) as a positive control. The HIF1α protein level in kidney was analyzed by immunohistochemistry.