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. 2008 Mar 14;105(12):4826–4831. doi: 10.1073/pnas.0712365105

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© 2008 by The National Academy of Sciences of the USA

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Fig. 3. — ATI triggers degradation of PML-RARα oncoprotein and reprogramming of differentiation regulators. (A) Enhanced degradation of PML-RARα is seen in NB4 (Left) and NB4-R2 (Right) cells treated with the ATI combination compared with those treated with mono- or bitreatment of A, T, and I. (B) TI intensifies matrix transfer from the nucleoplasm and ubiquitination of PML-RARα caused by A. Cells are pretreated with MG-132 for 1 h and then with the protocols indicated for 6 h. Western blot is performed as described (24). (C) Effects of ATI on differentiation regulators of NB4 cells at the protein level. (D) Effects of the ATI combination on differentiation regulators at the mRNA level, assessed by semiquantitative RT-PCR. (E) ATI treatment up-regulates the expression of RARβ2 at the mRNA level; this might be due to the dissociation of RARβ2 from HDAC1 upon ATI treatment.