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. 2008 Mar 14;105(12):4826–4831. doi: 10.1073/pnas.0712365105

Fig. 5.

Fig. 5.

T and I facilitate arsenic uptake by malignant promyelocytes via up-regulation of AQP9. (A) Combinatory use of T and/or I increases intracellular arsenic concentration ([As]i) in NB4 cells (*, P = 0.002; #, P = 0.004; **, P = 0.002). (B and C) T and/or I in combination with A up-regulate AQP9 expression at both the mRNA (B) and protein (C) levels (Coom, stained with Coomassie blue). (D) Immunofluorescence analysis of AQP9 expression in NB4 cells treated with the ATI combination. (E) AQP9-specific siRNA down-regulates AQP9 expression by approximately one-half in NB4 cells (NB4-AQP9-Si) compared with cells treated with nonsilencing siRNA control (NC). (F) Treatment with AQP9-specific siRNA reduces [As]i in NB4-AQP9-Si cells upon ATI compared with NB4-NC cells (*, P = 0.018; #, P = 0.037; **, P = 0.009). (G) Treatment with AQP9-specific siRNA inhibits differentiation of NB4 cells on ATI, revealed by analysis of CD11b expression.