Fig. 2.

Protein synthesis inhibitors induce PGC-1α in primary skeletal muscle cells. (A) Myotubes were treated with the indicated doses of anisomycin, emetine, or cycloheximide, and levels of PGC-1α mRNA were evaluated after 16 h. (B) Myotubes were treated with 200 ng/ml or 1 μg/ml of anisomycin or emetine for the indicated times, and PGC-1α mRNA was measured. (C) PGC-1α mRNA levels in myotubes treated with anisomycin or emetine after pretreatment for 30 min with actinomycin D. (D) Myotubes were treated with 30 ng/ml of anisomycin for 1–3 days, as indicated, and the mRNA levels of cycs, esrra, and cox5b were measured by qPCR.