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. 1996 Jul;34(7):1606–1609. doi: 10.1128/jcm.34.7.1606-1609.1996

Bicentric evaluation of Access Toxo immunoglobulin M (IgM) and IgG assays and IMx toxo IgM and IgG assays and comparison with Platelia Toxo IgM and IgG assays.

A Decoster 1, B Lecolier 1
PMCID: PMC229079  PMID: 8784554

Abstract

The recent Access immunoanalysis system (Sanofi Diagnostics Pasteur) for the serological diagnosis of toxoplasmosis was compared with the Abbott Toxo IMx EIA system, taking the Platelia Toxo immunoglobulin G (IgG) and Platelia Toxo IgM systems as references and using as confirmation methods an indirect fluorescence assay or a dye test for IgG and an immunosorbent agglutination assay (ISAGA) for IgM. A total of 1,461 serum samples were studied, of which 128 were collected from 42 recently seroconverted patients. Sensitivity and specificity rates of the Access system were 97.7 and 99.5%, respectively, for IgM and 98.6 and 100%, respectively, for IgG. Sensitivity and specificity rates of the Abbott IMx EIA system were 91 and 100%, respectively, for IgM and 92.5 and 100%, respectively, for IgG. The Access Toxo IgG and IgM EIA systems were found to be more sensitive than the Abbott Toxo IgG and IgM IMx EIA systems.

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Selected References

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  1. Cesbron J. Y., Caron A., Santoro F., Wattre P., Ovlaque G., Pierce R. J., Delagneau J. P., Capron A. Une nouvelle méthode ELISA pour le diagnostic de la toxoplasmose. Dosage des IgM sériques par immunocapture avec un anticorps monoclonal anti-Toxoplasma gondii. Presse Med. 1986 Apr 19;15(16):737–740. [PubMed] [Google Scholar]
  2. Dannemann B. R., Vaughan W. C., Thulliez P., Remington J. S. Differential agglutination test for diagnosis of recently acquired infection with Toxoplasma gondii. J Clin Microbiol. 1990 Sep;28(9):1928–1933. doi: 10.1128/jcm.28.9.1928-1933.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Desmonts G. Dépistage de la toxoplasmose par agglutination des parasites. Intérêt d'un antigène très sensible pour la recherche des immunoglobulines G spécifiques. Ann Biol Clin (Paris) 1983;41(2):139–143. [PubMed] [Google Scholar]
  4. FULTON J. D., TURK J. L. Direct agglutination test for Toxoplasma gondii. Lancet. 1959 Dec 12;2(7111):1068–1069. doi: 10.1016/s0140-6736(59)91535-1. [DOI] [PubMed] [Google Scholar]
  5. Franco E. L., Walls K. W., Sulzer A. J. Reverse enzyme immunoassay for detection of specific anti-Toxoplasma immunoglobulin M antibodies. J Clin Microbiol. 1981 May;13(5):859–864. doi: 10.1128/jcm.13.5.859-864.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GARIN J. P., AMBROISE-THOMAS P. LA DIAGNOSTIC S'EROLOGIQUE DE LA TOXOPLASMOSE PAR LA M'ETHODE DES ANTICORPS FLUORESCENTS (TECHNIQUE INDIRECTE) Presse Med. 1963 Dec 7;71:2485–2488. [PubMed] [Google Scholar]
  7. Gross U., Roos T., Appoldt D., Heesemann J. Improved serological diagnosis of Toxoplasma gondii infection by detection of immunoglobulin A (IgA) and IgM antibodies against P30 by using the immunoblot technique. J Clin Microbiol. 1992 Jun;30(6):1436–1441. doi: 10.1128/jcm.30.6.1436-1441.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hedman K., Lappalainen M., Seppäiä I., Mäkelä O. Recent primary toxoplasma infection indicated by a low avidity of specific IgG. J Infect Dis. 1989 Apr;159(4):736–740. doi: 10.1093/infdis/159.4.736. [DOI] [PubMed] [Google Scholar]
  9. Lecolier B., Pucheu B. Intérêt de l'étude de l'avidité des IgG pour le diagnostic de la toxoplasmose. Pathol Biol (Paris) 1993 Feb;41(2):155–158. [PubMed] [Google Scholar]
  10. Partanen P., Turunen H. J., Paasivuo R. T., Leinikki P. O. Immunoblot analysis of Toxoplasma gondii antigens by human immunoglobulins G, M, and A antibodies at different stages of infection. J Clin Microbiol. 1984 Jul;20(1):133–135. doi: 10.1128/jcm.20.1.133-135.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pinon J. M., Thoannes H., Gruson N. An enzyme-linked immuno-filtration assay used to compare infant and maternal antibody profiles in toxoplasmosis. J Immunol Methods. 1985 Feb 28;77(1):15–23. doi: 10.1016/0022-1759(85)90179-6. [DOI] [PubMed] [Google Scholar]
  12. Potasman I., Araujo F. G., Desmonts G., Remington J. S. Analysis of Toxoplasma gondii antigens recognized by human sera obtained before and after acute infection. J Infect Dis. 1986 Oct;154(4):650–657. doi: 10.1093/infdis/154.4.650. [DOI] [PubMed] [Google Scholar]
  13. Pouletty P., Kadouche J., Garcia-Gonzalez M., Mihaesco E., Desmonts G., Thulliez P., Thoannes H., Pinon J. M. An anti-human mu chain monoclonal antibody: use for detection of IgM antibodies to Toxoplasma gondii by reverse immunosorbent assay. J Immunol Methods. 1985 Feb 11;76(2):289–298. doi: 10.1016/0022-1759(85)90306-0. [DOI] [PubMed] [Google Scholar]
  14. Remington J. S. The present status of the IgM fluorescent antibody technique in the diagnosis of congenital toxoplasmosis. J Pediatr. 1969 Dec;75(6):1116–1124. doi: 10.1016/s0022-3476(69)80366-5. [DOI] [PubMed] [Google Scholar]
  15. Santoro F., Afchain D., Pierce R., Cesbron J. Y., Ovlaque G., Capron A. Serodiagnosis of toxoplasma infection using a purified parasite protein (P30). Clin Exp Immunol. 1985 Nov;62(2):262–269. [PMC free article] [PubMed] [Google Scholar]
  16. Schaefer L. E., Dyke J. W., Meglio F. D., Murray P. R., Crafts W., Niles A. C. Evaluation of microparticle enzyme immunoassays for immunoglobulins G and M to rubella virus and Toxoplasma gondii on the Abbott IMx automated analyzer. J Clin Microbiol. 1989 Nov;27(11):2410–2413. doi: 10.1128/jcm.27.11.2410-2413.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sharma S. D., Mullenax J., Araujo F. G., Erlich H. A., Remington J. S. Western Blot analysis of the antigens of Toxoplasma gondii recognized by human IgM and IgG antibodies. J Immunol. 1983 Aug;131(2):977–983. [PubMed] [Google Scholar]
  18. Turunen H., Vuorio K. A., Leinikki P. O. Determination of IgG, IgM and IgA antibody responses in human toxoplasmosis by enzyme-linked immunosorbent assay (ELISA). Scand J Infect Dis. 1983;15(3):307–311. doi: 10.3109/inf.1983.15.issue-3.12. [DOI] [PubMed] [Google Scholar]
  19. van Loon A. M., van der Logt J. T., Heessen F. W., van der Veen J. Enzyme-linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin M and A antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double-sandwich enzyme-linked immunosorbent assay. J Clin Microbiol. 1983 Jun;17(6):997–1004. doi: 10.1128/jcm.17.6.997-1004.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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