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. 2008 Mar 13;105(12):4709–4714. doi: 10.1073/pnas.0712018105

Fig. 3.

Fig. 3.

Analysis of methylation levels at promoter CpG islands in female hESCs in the presence or absence of XIST expression. (A) Bisulfite genomic sequencing analysis of the CpG island promoter and an exon region of CXORF12 gene in XIST+ and XIST− HSF6 cells. Note the promoter is 50% methylated in XIST+ HSF6 cells and becomes unmethylated in XIST− cells. In contrast, the exon region is 100% methylated in both XIST+ and XIST− cells. (B) Real-time quantitative PCR showing that the expression level of CXORF12 is significantly higher in XIST+ HSF6 cells compared with XIST− cells. *, P < 0.01. (C–E) Bisulfite methylation analysis in CpG islands of RBBP7, UTX, and CXORF15 genes. (F) Gene ontology analysis of 51 X-linked genes with decreased methylation levels in promoter CpG islands in XIST− hESCs (P < 0.05).